The effects of hypoxic preincubation on damage to bovine lung microvascular endothelial cells induced by subsequent hyperoxic exposure
Year: 2003
Type: article
Abstract: Objective The priming effects of hypoxia on intercellular adhesion molecule-1 (ICAM-1) expression in bovine lung microvascular endothelial cells (BLuECs) exposed to hyperoxic conditions were investigated． Methods After culture under normoxia or hypoxia (10Yo 02) for 72 hours， BLuECs were exposed to hyperoxia (50％ 02) with or without 5 ng／ml of tumor necrosis factor a (TNFcr)． Then the cell proliferation， LDH release， soluble ICAM-1 secretion， and the contents of cell-associated ICAM-1 were examined． Results There was no difference in cell proliferation in spite of differences in experimental conditions． Release of LDH was slightly increased at 24 hours after hyperoxic incubation in the hypoxia-hyperoxia group compared with the normoxia-hyperoxia group． Soluble ICAM-1 in media previously exposed to hypoxia showed a peak increase at 24 hours after exposure to hyperoxia and TNFα， although ICAM-l increased with time in the control culture media． Cytochalasin B inhibited this peak increase of soluble ICAM-L At 24 hours and at 48 hours after exposure to both hyperoxia and TNFcr， there was no difference in cell associated ICAM-1 between the culture with or without cytochalasin B． Conclusion Present results suggest that primed exposure to hypoxi a increases oxygen cytotoxicity and accelerates the release of ICAM-l fピom BLuECs exposed to both hyperoxia and TNFα． It is supposed that increased cytotoxicity following hypoxia may be involved in mechanism of acute lung injury often induced by oxygen supportive therapy．
Author: 竜 三神
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2273128752