Work

1H NMR-based urine metabolomics for the evaluation of kidney injury in Wistar rats by 3-MCPD

Year: 2016

Type: article

Abstract: H NMR, and the NMR spectrum signals of the detected metabolites were normalized and analyzed by orthogonal pattern recognition methods (PCA and OPLS-DA). This analysis revealed a time- and dose-dependency of the biochemical perturbations induced by 3-MCPD toxicity. Several metabolites responsible for glycine, serine and threonine metabolism, taurine and hypotaurine metabolism and nicotinate and nicotinamide metabolism revealed that 3-MCPD produced serious kidney toxicity, consistent with clinical biochemistry and histopathology. Significant changes in seven identified metabolites were validated as phenotypic biomarkers of 3-MCPD toxicity. Overall, our work demonstrates the powerful use of metabolomics for improved detection of toxicity and biomarker discovery and highlights the powerful predictive potential of such analyses for understanding food toxicity.

Source: Toxicology Research

DOI: 10.1039/c5tx00399g

Authors: Jian Ji, Lijuan Zhang, Hongxia Zhang, Chao Sun, Jiadi Sun, Hui Jiang, Mandour H. Abdalhai, YinZhi Zhang, Xiulan Sun

Institutions: Jiangnan University, State Key Laboratory of Food Science and Technology

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2274368136

FWCI: 1.28

Cites: 37

Cited by: 16

Related to: 10