Year: 2010
Type: article
Abstract: Coherent diffractive imaging of single particles using the single-shot ``diffract and destroy'' approach with an x-ray free electron laser (FEL) was recently demonstrated. A high-resolution low-noise coherent diffraction pattern, representative of the object before it turns into a plasma and explodes, results from the interaction of the FEL with the particle. Iterative phase retrieval algorithms are used to reconstruct two-dimensional projection images of the object from the recorded intensities alone. Here we describe the first single-shot diffraction data set that mimics the data proposed for obtaining 3D structure from identical particles. Ellipsoidal iron oxide nanoparticles ($250\text{ }\text{ }\mathrm{nm}\ifmmode\times\else\texttimes\fi{}50\text{ }\text{ }\mathrm{nm}$) were aerosolized and injected through an aerodynamic lens stack into a soft x-ray FEL. Particle orientation was not controlled with this injection method. We observed that, at the instant the x-ray pulse interacts with the particle, a snapshot of the particle's orientation is encoded in the diffraction pattern. The results give credence to one of the technical concepts of imaging individual nanometer and subnanometer-sized objects such as single molecules or larger clusters of molecules using hard x-ray FELs and will be used to help develop robust algorithms for determining particle orientations and 3D structure.
Institutions: SLAC National Accelerator Laboratory, Linac Coherent Light Source, Lawrence Livermore National Laboratory, Deutsches Elektronen-Synchrotron DESY, Center for Free-Electron Laser Science, Max Planck Institute for Medical Research, Uppsala University, Tofwerk (Switzerland), Lawrence Berkeley National Laboratory, Universität Hamburg
Language: English
Subfield: Radiation
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: diamond
Funders: U.S. Department of Energy, Eli Lilly and Company, Sven och Lilly Lawskis Fond för Naturvetenskaplig Forskning, Deutsche Forschungsgemeinschaft, Max-Planck-Gesellschaft, Vetenskapsrådet, Office of Science, Deutsches Elektronen-Synchrotron, Basic Energy Sciences, Laboratory Directed Research and Development, Lawrence Livermore National Laboratory, SLAC National Accelerator Laboratory
Awards: G1101028137, G1229526393, G1286236842, G1286251239, G129027128, G1356433355, G1414775356, G1445641779, G1451874870, G14554683, G1702950523, G1816284028, G213222014, G2145270357, G2173773851, G2371548206, G262532246, G303061748, G30714459, G3083819904, G3177268909, G3314968792, G365575497, G419881185, G4235786860, G4248083046, G42916606, G4501827968, G4744444288, G4908923434, G495301720, G498139845, G5570207115, G574795485, G5849692847, G628751139, G6445123630, G650090426, G6550344761, G7083997402, G7283024673, G7313278570, G765874931, G805243471, G8326222658, G8382000481, G911061179, G95070906, G969889393, G991281326
OpenAlex ID: W2293576019