ПОРУШЕННЯ ПРО-АНТИОКСИДАНТНОГО БАЛАНСУ ЯК МЕХАНіЗМ ВИНИКНЕННЯ ГЕНОТОКСИЧНИХ ПОШКОДЖЕНЬ В ОРГАНіЗМі ЗА ПОєДНАНОї Дії ЕКЗОГЕННИХ ОКСИДіВ АЗОТУ ТА МАЛИХ ДОЗ іОНіЗУЮЧОї РАДіАЦії ПРИ ПУХЛИННОМУ РОСТі
Year: 2013
Type: article
Abstract: The objective was to investigate the relationship between the intensity of free radical processes (FRP), activity of antioxidant enzymes and development of genetic instability in the organism in implementing genotoxic effects of exogenous nitric oxide (NO) and low doses of ionizing radiation (LDIR) in the process of tumor growth. Methods. The state of free radical processes in the blood was examined by a hydrogen peroxide-induced chemiluminescence (CL). The level of antioxidant enzymes was investigated using activities of superoxide dismutase (SOD) and catalase (CAT) in the rats blood. Genotoxicity were determined by alkaline horizontal gel electrophoresis of isolated peripheral blood lymphocytes. Results. Exogenous NO caused a profound imbalance of pro-and antioxidant processes accompanied by a long decline in the CL intensity comparatively to LDIR. Integral effect of oxidative metabolism disturbances under the joint action of NO and LDIR was due to NO. Changes in the activity of antioxidant enzymes in the blood after the action of NO and LDIR revealed a short-term decrease in CAT activity on the early stages of GC growth, while SOD activity remained decreased even at later stages of tumor growth especially under the influence of LDIR or NO + LDIR. Violation of pro-antioxidant balance in the organism leads to a significant increase in the amount of DNA strand breaks both under the action of NO and LDIR. Genotoxic effect of NO increased with tumor growth, and on 18-th day exceeded 2.9 – fold control level. Combined effect of NO and LDIR was accompanied by increase of lymphocytes DNA damage in 3.4-4 fold depending on the stage of GC growth. Thus, the combined effect of NO and/or LDIR causes the violation of the antioxidant balance in rats, which was accompanied by long-lasting genotoxic effect in peripheral blood lymphocytes with the increase of genetic damage in tumor bearing animals pretreated by these factors.
Source: Environment & Health
Language: Ukrainian
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2298044978