Rapid, Direct Quantification of Viruses in Solution Using the ViroCyt Virus Counter
Year: 2012
Type: article
Abstract: A direct virus quantification method that provides a rapid time to result and is well-correlated to standard virus quantification methods would be of high utility in a variety of research fields including bioprocessing and vaccine development. First, the measurement principles of the ViroCyt 2100 Virus Counter instrument will be introduced, highlighting the technological distinctions between this novel benchtop-sized flow cytometer and traditional flow cytometers. This will be followed by the presentation and discussion of data from several case studies and collaborative projects. Lastly, future technology development efforts will be discussed. Case studies and collaborative projects to be presented include the routine use of the Virus Counter to examine zero passage and first passage baculovirus viral stocks as an alternative to viral plaque assay, comparison of the Virus Counter and viral plaque assay for the examination of serial dilutions of viral stocks, comparative quantification of influenza virus using the Virus Counter and real-time reverse transcription polymerase chain reaction (RT-PCR), and direct monitoring of baculovirus growth in real-time in a suspension cell culture bioreactor as compared to traditional cell viability measurements. In each case presented, a correlation to the standard method will be examined, and any technological advantages of Virus Counter for these applications will be discussed.
Source: Europe PMC (PubMed Central)
Author: Erica D. Dawson
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2301764883