Work

Production of monoclonal antibodies reactive with ochratoxin A: Enzyme-linked immunosorbent assay for detection of ochratoxin A

Year: 1985

Type: article

Abstract: We have prepared seven mouse monoclonal antibodies (OTA.1, 2, 3, 4, 5, 6, 7) which react with ochratoxin A (OTA), a nephrotoxin produced by A. ochraceus. The OTA. 2 antibody equally reacted with OTA and ochratoxin B (OTB). Other antibodies (OTA.1, 3, 4, 5, 6, 7) reacted with OTA but not significantly with OTB. None of these antibodes cross-reacted significantly with ochratoxin α, coumarin and 4-hydroxycoumarin. The least detectable amount of OTA in the enzyme-linked immunosorbent assay with OTA.2 or OTA. 3 antibody was 5 to 50 pg OTA per assay. These monoclonal antibodies would be useful for the detection and quantitation of OTA in foods, feeds, and biological fluid.

Source: JSM Mycotoxins

DOI: 10.2520/myco1975.1985.28

Authors: J. CHIBA, H. KAJII, M. KAWAMURA, K. OHI, N. MOROOKA, Y. UENO

Institutions: Istituto Superiore di Sanità, National Institute of Health, National Institute of Health, Tokyo University of Science

Language: English

Topic: Mycotoxins in Agriculture and Food

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W2313809043

FWCI: 1.067

Cites: 1

Cited by: 6

Related to: 10