Year: 1956
Type: article
Abstract: 1. Glucose-6-phosphate was synthesized from glucose and tetraphosphoric acid by the method of SEEGMILLER and HORECKER. This compound was identified by the various analyses and also by the action of broad bean phosphoglucomutase which hereunto had not been reported in the paper of SEEGMILLER and HORECKER. 2. Glucose-6-phosphate was also prepared by the combined action of potato phosphorylase and broad bean phosphoglucomutase. Its reacting condition was as follows: inorganic phosphate, 0.05M; soluble starch, 1%; magnesium chloride, 0.005M; pH 7.5 and reaction temperature 30°. 3. From the result of this experiment, it was indicated that synthetic glucose-6-phosphate was more difficult to purify than that enzymatically prepared; the optical rotation and reducing value of the former preparation were slightly higher and the phosphorus content was slightly lower than the latter in its finally purified state. The difficulty of its purification may be eliminated when the condition of hydrolysis of the phosphorylated compound is improved.
Source: Nippon Nōgeikagaku Kaishi
Institution: Kyoto University
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2318366243