Work

Chloroplast «cryptic» promoter can be activated upon their transfer to plant nuclear genome

Year: 1996

Type: article

Abstract: It is shown that a DNA fragment containing the previously described exon 2 sequence of the chloroplast gene for ribosomal protein S12 can determine expression of the reporter npt-II gene intransgenic plant nuclear genome. Transcription start points inthetransgenic plant were localized within the rpS12 DNA coding sequences. After 5'-rpS12-CAT-nos-3' gene construction has been introduced to tobacco protoplasts by PEG-treatment, chloramphenicol acetyltransferase (CAT) enzyme activity was detectable by transient assays. These facts indicate that «cryptic» promoter-like sequences exist in chloroplast genome which can be activated as a result of their artifical or natural transfer to the nuclei.

Source: Biopolymers and Cell

DOI: 10.7124/bc.00045a

Authors: N. V. Grgzelyak, A. P. Galkin, L. V. Gening, T. V. Medvedeva, L. G. Lioshina, O. V. Bulko, K. G. Gasaryan

Institutions: National Academy of Sciences of Ukraine, Institute of Bioorganic Chemistry and Petrochemistry V.P. Kukhar, Institute of Molecular Genetics, Academy of Sciences of the Soviet Union

Language: English

Topic: Transgenic Plants and Applications

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W2320022095

FWCI: 0.408

Cites: 26

Cited by: 2

Related to: 10