Prospective Study of Diagnosis of Toxoplasma gondii Infection by Elisa and Real-time PCR in Immunocompromised Patients
Year: 2014
Type: article
Abstract: Event Abstract Back to Event Prospective Study of Diagnosis of Toxoplasma gondii Infection by Elisa and Real-time PCR in Immunocompromised Patients Hajira BERREDJEM1*, Imène BECHEKER1, Meriem BENLAIFA2, Maya Nadine BENSAID1, Karima BERREDJEM3, Rafika BARDI4 and Mohamed Reda DJEBAR2 1 University of Badji Mokhtar, Department of Biochemistry, Algeria 2 University of Badji Mokhtar, Department of Biology, Algeria 3 Dorban Hospital, Service of Hematology, Algeria 4 EPS Charles Nicolle, Tunisia Introduction : Toxoplasmosis is an infection of vertebrates caused by Toxoplasma gondii. It is one of the important opportunistic pathogen which has become an increasing problem worldwide due to the AIDS epidemic infection. Recent acquired or reactivated T.gondii infection is a serious complication in HIV patients. Classical serological diagnosis relies on the detection of anti-Toxoplasma immunoglobulin ; however, serology may be unreliable in HIV patients. PCR assays allow a rapid diagnosis of Toxoplasma infection. In this study, we investigated the value of the ELISA, the conventional PCR and the real-time PCR for diagnosing active toxoplasmosis in blood samples from HIV patients. Materials and Methods : IgG and IgM ELISA were used to determine anti-Toxoplasma antibodies titers in 25 blood samples from patients in whom 10 toxoplasmic encephalitis were suspected by clinical investigations. A conventional PCR and a real-time PCR (TaqMan assay) targeting the widely used B1 gene were performed to detecte T. gondii DNA. Results : Serological investigation did not contribute to confirm toxoplasmic infection, as IgG and IgM titers decreased early. 8 out 25 blood samples PCRs yielded a positive results for both conventional and real-time PCRs. However, the performance of real-time PCR was better than the conventional PCR one. The main advantages of Real-time PCR are that, it is performed in a closed system (the risks of contamination are minimal) and the result can be obtained in only 1 h. Furthermore, this study showed that it was possible to identify a high risk profile associated with an inceased parasitic charge. Conclusions : This study demonstrates the unusefulness of the serological tests and the high sensitivity and specificity of TaqMan PCR for the diagnosis of toxoplasmic encephalitis in HIV patients at an early stage wich facilitate effective treatment. Keywords: B1 gene, ELISA, HIV patients, Real-Time PCR, T. gondii. Conference: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014), Tlemcen, Algeria, 17 Oct - 20 Oct, 2014. Presentation Type: Poster Presentation Topic: Immunodeficiencies Citation: BERREDJEM H, BECHEKER I, BENLAIFA M, BENSAID M, BERREDJEM K, BARDI R and DJEBAR M (2014). Prospective Study of Diagnosis of Toxoplasma gondii Infection by Elisa and Real-time PCR in Immunocompromised Patients. Front. Immunol. Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014). doi: 10.3389/conf.fimmu.2014.04.00004 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Sep 2014; Published Online: 01 Dec 2014. * Correspondence: Dr. Hajira BERREDJEM, University of Badji Mokhtar, Department of Biochemistry, Annaba, 23000, Algeria, [email protected] Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hajira BERREDJEM Imène BECHEKER Meriem BENLAIFA Maya Nadine BENSAID Karima BERREDJEM Rafika BARDI Mohamed Reda DJEBAR Google Hajira BERREDJEM Imène BECHEKER Meriem BENLAIFA Maya Nadine BENSAID Karima BERREDJEM Rafika BARDI Mohamed Reda DJEBAR Google Scholar Hajira BERREDJEM Imène BECHEKER Meriem BENLAIFA Maya Nadine BENSAID Karima BERREDJEM Rafika BARDI Mohamed Reda DJEBAR PubMed Hajira BERREDJEM Imène BECHEKER Meriem BENLAIFA Maya Nadine BENSAID Karima BERREDJEM Rafika BARDI Mohamed Reda DJEBAR Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Source: Frontiers in Immunology
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W2320529316