Diagnosis of Koi Herpesvirus (KHV) Disease in Japan (Special Issue: International Symposium on Koi Herpesvirus disease)
Year: 2005
Type: article
Abstract: The koi herpesvirus (KHV) disease was designated as a “Specific Disease” in a Japanese law amended on June 30, 2003. The Ministry of Agriculture, Forestry and Fisheries officially announced the first case of KHV disease in cultured common carp Cyprinus carpio in Japan in early November 2003. At that time, the Prefectural Fisheries Experimental Stations (PFESs), which belong to local government, lacked sufficient equipment and skill required to conduct a polymerase chain reaction (PCR) tests for KHV detection. Therefore, at first, most PCR tests for KHV were carried out at the National Research Institute of Aquaculture (NRIA), Fisheries Research Agency (FRA). The NRIA gave a short training course of PCR for the research staff of PFESs in mid-November. According to the established guideline for the KHV disease, the PFESs now conduct epizootic and routine clinical examinations on diseased fish followed by PCR test to detect viral DNA in the tissues. When there is a positive reaction with the PCR test, the sample is sent to the NRIA for further confirmation by PCR. Virus isolation on the KF-1 cell line is also tried, but confirmatory diagnosis is based on the results of PCR tests. By the end of 2003, the NRIA has accepted 529 individuals of 87 cases to be diagnosed and KHV-infected carp have been found in 23 out of 47 prefectures.
Institution: National Agriculture and Food Research Organization
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2320898537