Work

A modified acidic approach for DNA extraction from plant species containing high levels of secondary metabolites

Year: 2014

Type: article

Abstract: Purified genomic DNA can be difficult to obtain from some plant species because of the presence of impurities such as polysaccharides, which are often co-extracted with DNA. In this study, we developed a fast, simple, and low-cost protocol for extracting DNA from plants containing high levels of secondary metabolites. This protocol does not require the use of volatile toxic reagents such as mercaptoethanol, chloroform, or phenol and allows the extraction of high-quality DNA from wild and cultivated tropical species.

Source: Genetics and Molecular Research

DOI: 10.4238/2014.august.25.13

Authors: M.M. Cavallari, M.V.B.M. Siqueira, T.M. Val, J.C. Pavanelli, M. Monteiro, C. Grando, J.B. Pinheiro, M.I. Zucchi, M.A. Gimenes

Institutions: Agência Paulista de Tecnologia dos Agronegócios, Universidade Sagrado Coração, Universidade de São Paulo, Universidade Estadual de Campinas (UNICAMP), Embrapa, Embrapa Recursos Genéticos e Biotecnologia

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Fundação de Amparo à Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico

OpenAlex ID: W2321907495

FWCI: 0.729

Cites: 14

Cited by: 13

Related to: 10