Work

The Immunodiagnosis of Drug-induced Allergic Hepatitis, by Macrophage Activating Factors Assay

Year: 1981

Type: article

Abstract: We performed a macrophage activating factors (MAF) assay, making use of the property that activated macrophages incorporate 3H-glucosamine, and applied it to clinical examination in the place of a macrophage migration inhibition assay.As a result of a fundamental experiment in which purified protein derivatives was taken as antigen, the suitable culture time of the macrophage was 3 days, and the optimal concentration of it was 1×106 cells/ml.Next, a MAF assay was performed under this condition in 10 cases against 12 drugs which showed the positive lymphocyte transformation of drug-induced allergic hepatitis. All cases showed the positive reactions (SI>200%), and the majority of the cases showed high SI values than those by whole blood micro-culture assay. MAF assay is easier than macrophage migration inhibition assay, and useful for the immuno-diagnosis of drug-induced allergic hepatitis.

Source: Japanese Journal of Clinical Immunology

DOI: 10.2177/jsci.4.129

Authors: Morikazu Onji, Yoshimasa Yamashita, Yasuyuki Ohta

Institution: Ehime University

Language: English

Topic: Drug-Induced Hepatotoxicity and Protection

Subfield: Pharmacology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W2322708488

FWCI:

Cites: 6

Cited by: 1

Related to: 10