Work

Identification of Japanese Flounder Leucocytes Involved in the Host Response to Neoheterobothrium hirame.

Year: 2003

Type: article

Abstract: Leucocyte types involved in tne cellular response of Japanese flounder Paralichthys olivaceus against the monogenean Neoheterobothrium hirame were identified. With electron microscopy, peripheral blood leucocytes (PBL) of Japanese flounder could be classified into four types : lymphocytes, thrombocytes, monocytes and granulocytes. Granulocytes were positive for acid phosphatase and peroxidase activities, and stained with Sudan black B. In fish infected with N. hirame, the ratios of granulocytes and monocytes significantly increased in the PBL composition. Infiltration of monocytes/macrophages and granulocytes, and cells having large electron-dense granules (DGC) was observed in the infection sites. These cells were often observed adhering to the tegument of N. hirame. Monocytes/macrophages showed particularly higher adherent ability to N. hirame than the other leucocytes under in vitro and in vivo conditions. We conclude that monocytes/macrophages, granulocytes and DGC are major effector cells in the defense of the flounder against N. hirame.

Source: Fish Pathology

DOI: 10.3147/jsfp.38.9

Authors: Chihaya Nakayasu, Nobuyuki Tsutsumi, Tomoyasu Yoshitomi, Tomoyoshi Yoshinaga, Akira Kumagai

Institutions: Japan Fisheries Research and Education Agency, The University of Tokyo, Public Works Research Institute, Miyagi Prefecture Freshwater Fisheries Experimental Station

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W2326528587

FWCI: 0.208

Cites: 24

Cited by: 10

Related to: 10