Seasonal changes of organophosphorous and organonitrogenous compounds hydrolase activities in Iidabori Pond.
Year: 1987
Type: article
Abstract: The activities of alkaline phosphatase, unease and protease were determined over a year in order to examine metabolic properties of microorganisms for nitrogenous and phosphorous nutrients in Iidabori Pond, a hypertrophic water body. Alkaline phosphatase activity in the unfiltered sample water increased in late May when algal bloom appeared for the first time, and during summer. The activity of the filtrate through a 0.45 μm membrane filter was 3% of that of the unfiltered sample water in summer. Two fractions with alkaline phosphatase activity were found on gel chromatography of phytoplankton extracts from Iidabori Pond. The approximate molecular weights were 400, 000 and 160, 000, respectively, and the latter was also found in the filtrate of the pond water.Urease activity was detected from early summer to autumn in the unfiltered water, but not in the filtrate. The protease activity in the unfiltered water reached maximum in summer and decreased from autumn to winter, but was not observed in the filtrate Judging from the dependence of three enzyme activities on pH and water temperature, the pond water was kept in suitable conditions for these hydrolases activities from early summer to autumn.
Authors: Masako TABATA, Mitsue IKEGAMI, Kazuko KUBOTA, Shizuo SUZUKI
Institution: Tokyo University of Science
Language: English
Subfield: Environmental Chemistry
Field: Environmental Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2331707587