Cytotoxic Effect of Hinokitiol and Tropolone on the Growth of Mammalian Cells and on Blastogenesis of Mouse Splenic T Cells.
Year: 1993
Type: article
Abstract: Hinokitiol (I) and tropolone (II) showed characteristic cytotoxic effects in vitro on five kinds of human and murine cell lines and blastic lymphocytes from mouse splenocytes. The cytotoxic effect of I on the growth of murine and human tumor cell lines, including RL male-1, MH134, HL60, K562 and KATO-III was definite when examined by thymidine incorporation into DNA and its 50% inhibitory concentration (IC50) on all cells was 0.3-0.6 microgram/ml. Compound II also showed comparable cytotoxic effects on these cell lines, indicating a little lower activity when compared to I. Furthermore, I and II also completely suppressed the [3H]thymidine ([3H]TdR) incorporation of mitogen-induced blastic lymphocytes. The suppressive activity on mouse lymphocyte proliferative response to concanavaline-A was also found with both compounds at a low concentration of 0.32 microgram/ml. As compound I is known to be of fairly low toxicity (LD50: 453 + 24 mg/kg in mice), the antitumor and immuno-suppressive effect of hinokitiol (I) should be further investigated.
DOI: 10.1248/bpb.16.521
Authors: Yoshihiko INAMORI, Hiroshi TSUJIBO, Hirofumi OHISHI, Fumio ISHII, Michinao MIZUGAKI, Hisashi ASO, Nakao ISHIDA
Institution: Osaka University of Pharmaceutical Sciences
Language: English
Subfield: Toxicology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2332495005