Work

Action mechanism of the novel rice blast fungicide tolprocarb distinct from that of conventional melanin biosynthesis inhibitors

Year: 2014

Type: article

Abstract: The target site of tolprocarb, a novel systemic fungicide used for controlling rice blast, was investigated. Tolprocarb decolorized the mycelia of Magnaporthe grisea; the decolorization was reversed by adding scytalone or 1,3,6,8-tetrahydroxynaphthalene (1,3,6,8-THN). This result suggested that the target site of tolprocarb was polyketide synthase (PKS), which regulated polyketide synthesis and pentaketide cyclization in melanin biosynthesis. Further, we produced a transgenic Aspergillus oryzae, which possessed the PKS gene of M. grisea, and performed in vitro assays of PKS using membrane fractions from the transgenic A. oryzae. Compared with some conventional melanin biosynthesis inhibitors (cMBIs), tolprocarb only inhibited PKS activity in vitro. These results indicated that tolprocarb’s target protein in M. grisea was PKS, which differentiates this fungicide from other cMBIs.

Source: Journal of Pesticide Science

DOI: 10.1584/jpestics.d14-033

Authors: Takahiro Hamada, Mineko Asanagi, Tomomi Satozawa, Natsuko Araki, Shinichi Banba, Norikazu Higashimura, Tomohisa Akase, Kangetsu Hirase

Institutions: Mitsui Chemicals Crop & Life Solutions, Inc (Japan), Japan Science and Technology Agency, Mitsui Chemicals (Japan)

Language: English

Topic: Fungal Plant Pathogen Control

Subfield: Ecology, Evolution, Behavior and Systematics

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

OpenAlex ID: W2332886407

FWCI: 2.739

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Cited by: 35

Related to: 10