Year: 2000
Type: article
Abstract: Summary: A high pressure freeze-substitution method was developed for immunoelectron microscopy(immuno-EM)of S. pombe. The method used a low concentration of OsO4 or uranyl acetate(UA)provided a high contrast in the specimen, while retaining the fine structure of the cells. In the image fixed with 0.01% OsO4-acetone, the cell wall and cytoskeleton were visible, while 0.1% UA-acetone was superior in the membranous structure and cytoplasm. The new method makes it possible to visualize the ultrastructure and localization of the material on one thin section for the immuno-EM.
Source: PLANT MORPHOLOGY
Authors: M. Onomi, N. Kamasawa, T. Takagi, M. Osumi
Institution: Japan Women's University
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2334479639