Brain-targeted delivery of resveratrol using solid lipid nanoparticles functionalized with apolipoprotein E
Year: 2016
Type: article
Abstract: BACKGROUND: The present study takes advantage of the beneficial effects of resveratrol as a neuroprotective compound. Resveratrol-loaded solid lipid nanoparticles were functionalized with apolipoprotein E which can be recognized by the LDL receptors overexpressed on the blood-brain barrier. RESULTS: Transmission electron microscopy images revealed spherical nanoparticles, dynamic light scattering gave a Z-average lower than 200 nm, and a zeta potential of around -13 mV and very high resveratrol entrapment efficiency (ca. 90 %). In vitro cytotoxic effects were assessed by MTT and LDH assays in hCMEC/D3 cell line and revealed no toxicity up to 50 μM over 4 h of incubation. The permeability through hCMEC/D3 monolayers showed a significant increase (1.8-fold higher) for resveratrol-loaded solid lipid nanoparticles functionalized with apolipoprotein E when compared to non-functionalized ones. CONCLUSIONS: In conclusion, these nanosystems might be a promising strategy for resveratrol delivery into the brain, while protecting it from degradation in the blood stream. Graphical abstract .
Source: Journal of Nanobiotechnology
Institutions: Universidade do Porto, Rede de Química e Tecnologia
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,790
Awards: G2045472859, G2282515878, G2649847610, G2673245850, G2711267513, Unidade de Ciências Biomoleculares Aplicadas, G3008461997, A MEMBRANE BIOPHYSICAL APPROACH TO THE THERAPEUTIC EFFECTS OF RESVERATROL AND DESIGN OF NOVEL NUTRACEUTICAL NANOFORMULATIONS, G33681276, G384318868, G3860734975, G3902363903, G875854470, G8839327758, G8902177905, G929281750
OpenAlex ID: W2335645192