Isolation and Identification of Cellulolytic and Xylanolytic Bacteria from Huancarhuaz Hot Spring, Peru
Year: 2014
Type: article
Abstract: Aims: To isolate and characterize lignocellulase producing thermophilic bacteria from a Peruvian hot spring.Study Design: Combined sediment and water samples from the hot spring were subjected to direct plating, in situ baiting and ex situ enrichment.Endoglucanase and xylanase producing bacterial colonies were isolated and characterized.Place and Duration of Study: Samples were taken from the Huancarhuaz hot spring, Peru (8º56'31.86"S,77º47'00.53"W) in August 2010 and processed during 2011-2013.Methodology: Samples were subjected to three isolation methods and bacterial colonies with different color, size and appearance, were isolated, purified by streaking several times and conserved in Tryptic Soy Agar slants at 4ºC.The agar staining method was used to isolate enzyme-producing strains which were then identified by 16S rRNA sequencing and further studied for endoglucanase and xylanase production.
Author: Carmen Tamariz-Angeles
Institution: Universidad Nacional Santiago Antúnez de Mayolo
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2336497352