A biodegradable knitted cardiac patch for myocardium regeneration using cardiosphere-derived cells (CDCs)
Year: 2016
Type: article
Abstract: Event Abstract Back to Event A biodegradable knitted cardiac patch for myocardium regeneration using cardiosphere-derived cells (CDCs) Jiyang Chen1*, Andre J. West2, Ke Cheng1 and Martin W. King1, 3 1 North Carolina State University, College of Textiles, United States 2 University of North Carolina & North Carolina State University, College of Veterinary Medicine and UNC - NCSU Joint Department of Biomedical Engineering, United States 3 Donghua University, College of Textiles, China Introduction: Nowadays, the recovery of damaged myocardium for patients who survive an acute myocardial infarction (MI) is to use tissue engineering therapies to regenerate myocardial tissue by delivering stem cells directly to the scar site. However, direct injection has an extremely low cell survival rate[1]. So the use of a cardiac patch to prevent cells from being dispersed and to promote cell attachment and proliferation is an attractive alternative strategy[2]. In addition, the cardiac patch can provide mechanical support to the ventricular wall while it experiences remodeling. Today the key remaining question is how to design a cardiac patch that can mimic the non-linear mechanical properties of human myocardium. The present study attempts to answer this question by designing weft knitted fabrics that can match the compliance and recovery of myocardium[3]. For the cell line, cardiosphere-derived stem cells (CDCs) were selected since they have previously demonstrated improved left ventricular function following direct injection in rats[4]. Materials and Methods: In a preliminary study, various prototype textile structures were weft knitted from polyester (polyethylene terephthalate) (PET) yarns, and their thickness, mass per unit area and mechanical properties were measured to determine the optimal structure for further evaluation as a cardiac patch knitted from resorbable polylactic acid (PLA) yarns. Uniaxial and biaxial tests were applied to determine the mechanical properties of the patch structures. The CDCs were seeded and cultured on the PLA samples for seven days followed by SEM observation and an MTT assay. Results: The initial tensile modulus of a PET tuck stitch structure was similar to that of human myocardium. A PET rib stitch structure had the highest total porosity (Figure 1), while a PET jersey stitch structure had the lowest.These three patch structures were selected to fabricate PLA samples. Fig 1.Rib stitch structure In subsequent uniaxial and biaxial tensile tests, the tuck and rib stitch structures gave stress-strain curves and Young’s moduli that were similar to human and rat myocardium. The jersey stitch produced the stiffest fabric, and for the biological test after 7 days the MTT proliferation assay absorbance was higher for the rib structure than the other two patch samples (Figure 2). Fig 2. Results of MTT assay Discussion: Following mechanical testing both the jersey and rib structures mimicked the non-linear mechanical properties of myocardium. From the in vitro cell culture testing the scanning electron microscopy (SEM) confirmed the attachment and proliferation of the CDCs on the surface of the PLA fibers. Conclusion: The rib stitch structure was found to be the most promising choice for the design of a PLA knitted cardiac patch. Further animal studies will be needed to demonstrate that a textile cardiac patch weft knitted from PLA yarns can function successfully in vivo. Dr.Hsiao-Ying Shadow Huang; Taylor Hensley; Bryan DanleyReferences:[1] Laflamme, M. A., & Murry, C. E. (2005). Regenerating the heart. Nature Biotechnology, 23(7), 845-856.[2] Fujimoto, K. L., Guan, J., Oshima, H., Sakai, T. & Wagner, W. R. (2007). In vivo evaluation of a porous elastic biodegradable patch for reconstructive cardiac procedures. Annals of Thoracic Surgery, 83(2), 648-654.[3] Boublik, J., Park, H., Radisic, M., Tognana, E., Chen, F., Pei, M. & Freed, L. E. (2005). Mechanical properties and remodeling of hybrid cardiac constructs made from heart cells, fibrin, and biodegradable, elastomeric knitted fabric. Tissue Engineering, 11(7-8), 1122-1132.[4] Makkar, R. R., Smith, R. R., Cheng, K., Malliaras, K., Thomson, L. E., Berman, D. & Marbán, E. (2012). Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial. The Lancet, 379(9819), 895-904. Keywords: Tissue Regeneration, mechanical property, Biodegradable material, Heart repair Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Chen J, West AJ, Cheng K and King MW (2016). A biodegradable knitted cardiac patch for myocardium regeneration using cardiosphere-derived cells (CDCs). Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02002 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Jiyang Chen, North Carolina State University, College of Textiles, Raleigh, NC, United States, Email1 Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jiyang Chen Andre J West Ke Cheng Martin W King Google Jiyang Chen Andre J West Ke Cheng Martin W King Google Scholar Jiyang Chen Andre J West Ke Cheng Martin W King PubMed Jiyang Chen Andre J West Ke Cheng Martin W King Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Authors: Chen Jiyang, West Andre, Cheng Ke, King Martin
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W2336668648