Work

Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Year: 2016

Type: article

Abstract: The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.

Source: Scientific Reports

DOI: 10.1038/srep24484

Authors: Michihiro Sugahara, Changyong Song, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Takanori Nakane, Fumiaki Yumoto, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, So Iwata

Institutions: SPring-8, RIKEN, Pohang University of Science and Technology, Institute for Protein Research, The University of Osaka, The University of Osaka, Kyoto University, The University of Tokyo, High Energy Accelerator Research Organization, Japan Synchrotron Radiation Research Institute, RIKEN Center for Sustainable Resource Science

Language: English

Topic: Enzyme Structure and Function

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,690

Funders: Ministry of Education, Culture, Sports, Science and Technology, Japan Society for the Promotion of Science, Japan Science and Technology Agency

Award: Protein crystallization method in gel capsules with nucleants for contactless handling of crystals

OpenAlex ID: W2338845686

FWCI: 4.802

Cites: 35

Cited by: 59

Related to: 10