Year: 1975
Type: article
Abstract: In tro d u ctio n Tissue culture of M eteor pea was obtained in Ju ly 1968 from the proliferation of an im m ature em bryo.The tissue w as isolated in the search for a system w hich m ight be useful in the study of gibberellin action at the level of unorganized cells.D uring the prelim inary routine experim ents aim ed at finding the optim al culture medium, it was noted th a t the tissue was ra th e r specific in its requirem ents for grow th.The presen t paper reports the observations concerning the effect of m ineral salts, carbohydrates, vitam ins and hormones.Callus tissues of different pea varieties have not been very often used in studies of n u tritio n and morphogenesis.T o r r e y and S h i g em u r a (1957) obtained a callus tissue from A laska pea roots using a m odified B onner m ineral medium, w ith sucrose, auxin and vitam ins.The root-form ing capacity of several strains of th a t tissue was studied la te r on in relation to chromosomal constitution (T o r r e y, 1967).The production of cytokinins was investigated by using higher salt concen tratio n s and a m ore complex m ixture of vitam ins and amino acids (Short and T o r r e y, 1972).H ild e b ra n d t e t a l .(1963) isolated a callus from th e stem tissue, w hich was grow ing on W hite m ineral solution, sucrose, NAA, inositol and casein hydrolysate.Bailey (1970) studied th e production of pisatin by pea tissue cultivated on a sim ilar medium, containing vitam ins, glycine, 2,4-D and coconut milk.K a 11 a k and Y arvekylg (1971) investigated the cytogenetic effect of 2,4-D in a pea callus, derived from cotyledons and grow n on the T orrey m e dium.The influence of environm ental conditions, nitrogen nutrition and horm ones on shoot form ation in a callus tissue obtained from excised field pea apices was studied by G a m b o r g et al. (1974).A pparently, pea callus tissue can satisfactorily be grow n on various m edia and the precise n u tritio n al requirem ents w ere not studied in m ost cases.
Authors: Nešković, Mirjana, Radojević, Ljiljana
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2394937696