A Wash-Free Homogeneous Colorimetric Immunoassay Method
Year: 2015
Type: article
Abstract: Rapid and convenient biosensing platforms could be beneficial to timely diagnosis and treatment of diseases in virtually any care settings. Sandwich immunoassays, the most commonly used methods for protein detection, often rely on expensive tags such as enzyme and tedious wash and incubation procedures operated by skilled labor. In this report, we revolutionized traditional sandwich immunoassays by providing a wash-free homogeneous colorimetric immunoassay method without requirement of any separation steps. The proposed strategy was realized by controlling the growth of gold nanoparticles (AuNPs) to mediate the interparticle spacing in the protein-AuNP oligomers. We have demonstrated the successful in vitro detection of cancer biomarker in serum samples from patients with high clinical sensitivity and specificity.
Source: Theranostics
DOI: 10.7150/thno.13159
Authors: Huiqiao Liu, Pengfei Rong, Hongwei Jia, Jie Yang, Bo Dong, Qiong Dong, Cejun Yang, Pengzhi Hu, Wei Wang, Haitao Liu, Dingbin Liu
Language: English
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,671
OpenAlex ID: W2396773151