Molecular Cloning and Characterization of a 2-Deoxystreptamine Biosynthetic Gene Cluster in Gentamicin-producing Micromonospora echinospora ATCC15835
Year: 2004
Type: article
Abstract: The organization of the 2-deoxystreptamine (DOS) biosynthetic gene cluster of Micromonospora echinospora has been determined. Sequencing of a 14.04 kb-region revealed twelve open reading frames (ORFs): four putative DOS biosynthetic genes (gtmA, B, C, and D), five amino sugars biosynthetic genes (gtmE, G, H, I, and gacB), two aminoglycoside resistance genes (gtmF and J) as well as a hypothetical ORF (gacA). One of the putative DOS biosynthetic genes, gtmA, was expressed in Escherichia coli, and the purified protein was shown to convert glucose-6-phosphate (G-6-P) to 2-deoxy-scyllo-inosose (DOI), a key step in DOS biosynthesis. In addition gtmJ was expressed in Streptomyces lividans and shown to confer gentamicin resistance. Thus gtmA and gtmJ are implicated in the biosynthesis of gentamicin and in resistance to it, respectively.
Source: Molecules and Cells
Authors: Madan K. Kharel, Devi B. Basnet, Hei Chan Lee, Kwangkyoung Liou, Young Ho Moon, Jae-Jong Kim, Jin Suk Woo, Jae Kyung Sohng
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $2,500
OpenAlex ID: W2399257646