Anti-aging effects of Lactobacillus gasseri SBT2055 and the mechanism of action
Year: 2016
Type: article
Abstract: Aim. The beneficial effects of Lactobacillus gasseri SBT2055 (LG2055) on animal and human health have been reported. In this study, we first investigated whether LG2055 has the anti-aging effects on Caenorhabditis elegans (C. elegans) and mammalian cell. Methods. We analyzed the life span after administration of LG2055 or control E.coli OP50 in wild type N2 and several mutants of C. elegans. In addition, expression of critical genes for oxidative stress response was determined using qPCR. Furthermore, we examined the influence of LG2055 treatment on the mitochondria membrane potential, total ATP levels and mitochondria mass in mammalian cell damaged by CCCP (Carbonyl cyanide m-chlorophenyl hydrazone). Results. LG2055 feeding up-regulated the expression of skn-1 gene and its target genes. Interestingly, mitochondrial mass and ATP level were significantly increased by LG2055 feeding in comparison with the control group. In mammalian cell, CCCP decreased the number of mitochondria, but LG2055 treatment inhibited the decrease of mitochondrial mass. LG2055 also enhanced the function of mitochondria, increased ATP levels and decreased ROS levels. Conclusion. LG2055 feeding/treatment were effective to extend lifespan by strengthening resistance to the oxidative stress and up-regulation of the mass and function of mitochondria in C.elegans and mammalian cell.
Language: English
Subfield: Aging
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2415423219