Deoxyribonucleic Acid Synthesis in Bacteriophage SP01-infected Bacillus subtilis
Year: 1973
Type: article
Abstract: A new DNA polymerase has been purified 1000-fold from Bacillus subtilis infected with bacteriophage SP01. It consists of a single polypeptide chain of approximately 122,000 molecular weight. The enzyme requires deoxyribonucleoside 5'-triphosphates, Mg2+, β-mercaptoethanol, and single stranded DNA templates for activity. Bihelical DNA which has been partially denatured serves as the preferred template in in vitro reactions. Although 5-hydroxymethyluracil replaces thymine in SP01 DNA, deoxynucleotide analogues of 5-hydroxymethyldeoxyuridine 5'-triphosphate can serve as efficient substrates for the phage polymerase. The purified enzyme is free of endonuclease activity.
Source: Journal of Biological Chemistry
Authors: Clifford O. Yehle, A.T. Ganesan
Institutions: Stanford Medicine, Stanford University
Language: English
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
OpenAlex ID: W24348274