Characterizing the Influence of Neurotrophic Factors on Regenerating Neurites - An in-vitro Neurite Regeneration Model using a Microfluidic-Microelectronic Platform
Year: 2016
Type: article
Abstract: Event Abstract Back to Event Characterizing the Influence of Neurotrophic Factors on Regenerating Neurites - An in-vitro Neurite Regeneration Model using a Microfluidic-Microelectronic Platform Patrick Schuller1, Martina Gergits1, Johann K. Mika1, Heinz Wanzenboeck1*, Petra Scholze2 and Emmerich Bertagnolli1 1 Vienna University of Technology, Institute of Solid State Electronics, Austria 2 Medical University of Vienna, Center for Brain Research, Austria Motivation The understanding of how neurites of nerve cells are guided to each other plays an important role in neurobiology inter alia in regrowth and repair of disconnected nerve cells. During the last decades, different neurotrophic factors have been identified which are essential for the survival and growth of neurites [1]. In order to investigate the regeneration behavior of neurites of CNS and PNS neurites under the influence of neurotrophic factors in vitro we developed a microfluidic-microelectronic platform, which enables the culture of these cells and the separation of the growing processes from the somas. Material and Methods A microfluidic platform was attached on top of a microelectrode array (featuring 60 microelectrodes) [2]. The designs of both components are matched to each other and can be adapted to the experiment. The microfluidic platform consists of two separated culture chambers connected with 15 to 60 microchannels. In the presented work the length of microchannels was 100-1000 µm and the cross-section was 5 10 x 4 µm². This cross-section prevents cell somata from entering so that growing neurites can be investigated isolated from the somata. Rodent CNS and PNS nerve cells are seeded in the culture chamber and are cultivated for up to 28 days in medium containing different concentrations of neurotrophins. Figure 1 shows a volume rendered image of SCG cells in the culture chamber attached to the MEA, created from confocal laser microscopy images. Discussion We observed the formation of neurites from HC, SCG and PC12 cells using electrophysiological measurements as well as immunocytochemistry for up to four weeks. For the neuronal characterization different parameters, such as interspike-interval and propagation velocity, of the regenerating nerve cells were calculated. Furthermore, optimal concentrations of specific neurotrophic factors such as NGF could be identified. Figure 2 shows the influence of NGF on the regeneration of neurites from SCG cells over 2 weeks. Conclusion The capability for electrophysiological tracking of the development of regenerating neurites enables to study the combination of different neurotrophins. The developed microfluidic-microelectronic platform will help to understand the connection between neurotrophic factors under standardized test conditions. References [1] Binan L, Ajji A, De Crescenzo G, Jolicoeur M. Approaches for Neural Tissue Regeneration. Stem Cell Rev and Rep. 2014;10:44-59. [2] Mika JK, Schwarz K, Wanzenboeck HD, Scholze P, Bertagnolli E. Investigation of neurotrophic factor concentrations with a novel in vitro concept for peripheral nerve regeneration. Journal of neuroscience research. 2015;93:1631-40. Figure Legend Figure 1: Volume rendered image of SCG cells in the culture chamber attached to the MEA. Figure 2: Influence of different concentrations of NGF on the regeneration of neurites over time. Figure 1 Keywords: Microfluidics, MEA, neurite, SCG, neurotrophine Conference: MEA Meeting 2016 | 10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany, 28 Jun - 1 Jul, 2016. Presentation Type: Poster Presentation Topic: MEA Meeting 2016 Citation: Schuller P, Gergits M, Mika JK, Wanzenboeck H, Scholze P and Bertagnolli E (2016). Characterizing the Influence of Neurotrophic Factors on Regenerating Neurites - An in-vitro Neurite Regeneration Model using a Microfluidic-Microelectronic Platform. Front. Neurosci. Conference Abstract: MEA Meeting 2016 | 10th International Meeting on Substrate-Integrated Electrode Arrays. doi: 10.3389/conf.fnins.2016.93.00105 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Jun 2016; Published Online: 24 Jun 2016. * Correspondence: Dr. Heinz Wanzenboeck, Vienna University of Technology, Institute of Solid State Electronics, Vienna, Austria, [email protected] Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Patrick Schuller Martina Gergits Johann K Mika Heinz Wanzenboeck Petra Scholze Emmerich Bertagnolli Google Patrick Schuller Martina Gergits Johann K Mika Heinz Wanzenboeck Petra Scholze Emmerich Bertagnolli Google Scholar Patrick Schuller Martina Gergits Johann K Mika Heinz Wanzenboeck Petra Scholze Emmerich Bertagnolli PubMed Patrick Schuller Martina Gergits Johann K Mika Heinz Wanzenboeck Petra Scholze Emmerich Bertagnolli Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Source: Frontiers in Neuroscience
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W2467257101