Year: 2016
Type: article
Abstract: Lateral root (LR) emergence represents a highly coordinated process in which the plant hormone auxin plays a central role. Reactive oxygen species (ROS) have been proposed to function as important signals during auxin-regulated LR formation; however, their mode of action is poorly understood. Here, we report that Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP). Strikingly, ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines in which auxin-mediated cell wall accommodation and remodeling in cells overlying the sites of LR formation is disrupted. Specifically, ROS are deposited in the apoplast of these cells during LR emergence, following a spatiotemporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH). We also show that disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs. We conclude that RBOH-mediated ROS production facilitates LR outgrowth by promoting cell wall remodeling of overlying parental tissues.
Source: Development
DOI: 10.1242/dev.136465
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
Funders: European Commission, Fonds De La Recherche Scientifique - FNRS, Belgian Federal Science Policy Office, Fédération Wallonie-Bruxelles, Fonds Wetenschappelijk Onderzoek, Universiteit Gent, Vlaamse regering, Biotechnology and Biological Sciences Research Council, Fonds pour la Formation à la Recherche dans l’Industrie et dans l’Agriculture, Bijzonder Onderzoeksfonds UGent, FP7 Food, Agriculture and Fisheries, Biotechnology
Awards: Hydropatterning, G1976482657, G3394992377, Characterisation of the molecular and cellular mechanisms controlling lateral root emergence using an integrative-systems based approach, G484229801, Root SAT-NAV: uncovering the molecular mechanisms guiding root angle in soil, Engineering root architecture using a predictive integrative systems biology approach, Evolution of the plant lateral root initiation process, G7582053920, Growth and development of higher plants (MARS).
OpenAlex ID: W2472226136