Work

Use of Isozyme Techniques to Identify Peach x ‘Nonpareil’ Almond Hybrids

Year: 1987

Type: article

Abstract: Two isozyme systems, phosphoglucomutase (PGM) and 6-phosphogluconate dehydrogenase (PGD), were identified that permit the early verification of peach x almond hybrids as opposed to plants resulting from accidental self-pollination. Nondestructive samples for analysis can be taken from cotyledons or primary leaves. Isozymes of PGM-2, PGD-1, and PGD-2 were found to be monomorphic in the peach [ Prunus persica (L.) Batsch] cultivars studied. P. amygdalus Batsch ‘Nonpareil’ was found to be heterozygous at the PGM-2 banding region, bearing the peach allele and a slower migrating allele. This locus allows verification of 50% of the hybrids. ‘Nonpareil’ was homozygous for alleles contrasting to peach at the PGD-1 and PGD-2 banding regions, allowing the unequivocal verification of all hybrids.

Source: HortScience

DOI: 10.21273/hortsci.22.2.300

Authors: J. X. Chaparro, R. E. Durham, G. A. Moore, W. B. Sherman

Institutions: University of Florida, Institute of Food and Agricultural Sciences

Language: English

Topic: Genetic Mapping and Diversity in Plants and Animals

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,000