Work

Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models

Year: 2016

Type: article

Abstract: Veterinaria México OA ISSN: 2448-6760Cite this as:Ibarra Zazueta C, Arellano Reynoso B, Hernández Castro R, Palomares Resendiz EG, Diaz Aparicio E. Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. Veterinaria México OA. 2016;3(4). doi: 10.21753/vmoa.3.4.366Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis. The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14th day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. Hence, this study contributes to the knowledge of the immune response against Corynebacterium pseudotuberculosis.

Source: Veterinaria

DOI: 10.21753/vmoa.3.4.366

Authors: Cristina Ibarra Zazueta, Beatriz Arellano Reynoso, Rigoberto Hernández Castro, Erika Gabriela Palomares Resendiz, Efren Diaz Aparicio

Institutions: Universidad Nacional Autónoma de México, Hospital General Dr. Manuel Gea Gonzalez, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias

Language: English

Topic: Diphtheria, Corynebacterium, and Tetanus

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Consejo Nacional de Ciencia y Tecnología