Work

Purification and Characterization of Cellulase from Termite Ametermes eveuncifer (Silverstri) Soldiers

Year: 2016

Type: article

Abstract: Cellulase enzyme was purified and characterized from termite soldiers (Ametermes eveuncifer) using 70% ammonium sulphate precipitation, ion exchange chromatography and affinity chromatography. The enzyme isolated had a specific activity of 5.04 U/mg with a percentage yield of 11.7%. The enzyme showed maximum activity at 500C and pH 8. The enzyme was not inhibited by Ba2+ at a concentration of 1mM and Pb2+ at 10 mM concentration but was inhibited by other metal ions at 1 mM and 10 mM concentrations of their salts (NaCl, KCl, MnCl2, and NiCl2),

Source: International Journal of Biology

DOI: 10.5539/ijb.v9n1p1

Authors: B. S. Fagbohunka, R. E. Okonji, Ayinla Zainab Adenike

Institutions: Olabisi Onabanjo University, Ekiti State University, Adekunle Ajasin University, Obafemi Awolowo University

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W2561951981

FWCI: 0.873

Cites: 34

Cited by: 12

Related to: 10