Year: 2016
Type: preprint
Abstract: RNA polymerase V (Pol V) long noncoding RNAs (lncRNAs) have been proposed to guide ARGONAUTE4 (AGO4) to chromatin in RNA-directed DNA methylation (RdDM) in plants. Here, we provide evidence, based on laser UV-assisted zero-length cross-linking, for functionally relevant AGO4-DNA interaction at RdDM targets. We further demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine-tryptophan/tryptophan-glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L. We propose a model in which AGO4-DNA interaction could be responsible for the unique specificities of RdDM.
Source: Genes & Development
Institutions: Centre National de la Recherche Scientifique, Université de Perpignan, Laboratoire Génome et Développement des Plantes, University of California, Los Angeles, Broad Center, University of Washington, Inserm, Institut pour l'avancée des biosciences, Université Grenoble Alpes, Lyon 1 Université, École Normale Supérieure de Lyon, Laboratoire de Biologie et Modélisation de la Cellule, Institut NeuroMyoGène, Howard Hughes Medical Institute
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
Awards: Functional analysis of a new siRNA-dependent transcriptional gene silencing pathway that targets rapidly evolving genomic regions of the plant genome., G2973790809, G3207356259, G393177772, The Argonaute hook motif: its function in RNA silencing in Arabidopsis and viral/bacterial mechanisms of evasion in plants., Genetics of DNA methylation patterning of arabidopsis., G5702163051, Genetics of DNA Methylation Patterning of Arabidopsis, G9000078557
OpenAlex ID: W2565097302