Naa50/San-dependent N-terminal acetylation of Scc1 is potentially important for sister chromatid cohesion
Year: 2016
Type: article
Abstract: The gene separation anxiety (san) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of this enzyme is still poorly understood. Naa50/San was originally proposed to be required for centromeric sister chromatid cohesion in Drosophila and human cells, yet, more recently, it was also suggested to be a negative regulator of microtubule polymerization through internal acetylation of beta Tubulin. We used genetic and biochemical approaches to clarify the function of Naa50/San during development. Our work suggests that Naa50/San is required during tissue proliferation for the correct interaction between the cohesin subunits Scc1 and Smc3. Our results also suggest a working model where Naa50/San N-terminally acetylates the nascent Scc1 polypeptide, and that this co-translational modification is subsequently required for the establishment and/or maintenance of sister chromatid cohesion.
Source: Scientific Reports
DOI: 10.1038/srep39118
Authors: Ana Luisa Ribeiro, Rui D. Silva, Håvard Foyn, Margarida N. Tiago, Om Singh Rathore, Thomas Arnesen, Rui Gonçalo Martinho
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: Drosophila Transgenic RNAi Resource Project, G1897079340, Segregação de proteínas e acetilação N-terminal, G2461055997, Obstáculos moleculares ao processamento de pre-mRNAs durante uma rápida proliferação celular., REGULATION OF PROTEIN N-TERMINAL ACETYLATION DURING DROSOPHILA DEVELOPMENT, G5432930453, G6222146311, REGULATION OF PRE-MRNA SPLICING DURING DROSOPHILA DEVELOPMENT, G6747835480, G6878737446, G8514436827
OpenAlex ID: W2567071082