Work

MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold

Year: 2017

Type: article

Abstract: The effect of dynamic compressive stimulation on MG-63 cell proliferation on an auxetic PLGA scaffold was investigated. The estimated Poisson ratio of the prepared auxetic scaffold specimens was approximately (−)0.07, while the Poisson ratio estimated for conventional scaffold specimens was (+)0.12 under 10% strain compression on average. Three stimulus groups were examined: control (no stimulation), static compression, and dynamic compression. In preparation for proliferation testing, cells were seeded at 2.2 × 105 cells/80 μL on each scaffold specimen. The average proliferation rates of the static and dynamic groups were higher than those of the control group: 13.4% and 25.5% higher at culture day 1, 34.7% and 56.2% at culture day 3, and 17.5% and 43.0% at culture day 5, respectively. The static and dynamic group results at culture day 5 were significantly different ( p<0.01 ). Moreover, proliferation rate of the dynamic stimulation group was 1.22 times higher than that of the static group ( p<0.01 ). Conclusively, proliferation of osteoblast-like cells was enhanced through compressive stimulation, but the enhancement was maximal with dynamic compressive stimulation of auxetic scaffolds.

Source: International Journal of Polymer Science

DOI: 10.1155/2017/1286109

Authors: Myeong Jin Kim, Hong Jin Choi, Jongman Cho, Jung Bok Lee, Hak-Joon Sung, Jeong Koo Kim

Institutions: Inje University, Vanderbilt University

Language: English

Topic: Bone Tissue Engineering Materials

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,160