Year: 2017
Type: article
Abstract: In mammals, the master circadian clock is located in the suprachiasmatic nucleus (SCN), where most neurons show circadian rhythms of intracellular Ca2+ levels. However, the origin of these Ca2+ rhythms remains largely unknown. In this study, we successfully monitored the intracellular circadian Ca2+ rhythms together with the circadian PER2 and firing rhythms in a single SCN slice ex vivo, which enabled us to explore the origins. The phase relation between the circadian PER2 and Ca2+ rhythms, but not between the circadian PER2 and firing rhythms, was significantly altered in Cry1/Cry2 double knockout mice, which display a loss of intercellular synchronization in the SCN. In addition, in Cry1/Cry2 double knockout mice, circadian Ca2+ rhythms were abolished in the dorsolateral SCN, but were maintained in the majority of the ventromedial SCN. These findings indicate that intracellular circadian Ca2+ rhythms are composed of an exogenous and endogenous component involving PER2 expression.
Source: Scientific Reports
DOI: 10.1038/srep41733
Institutions: Hokkaido University, Japan Science and Technology Agency
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: Neuronal network level understanding of master circadian clock in mammals, G2926096991, Understanding of circadian system in the central circadian clock using in vivo recording, Measurement of transcription activity in freely moving mice: Ultradian rhythms and their physiological roles, Light entrainment mechanism in the suprachiasmatic nucleus by muti-functional optical imaging and cell function manipulation, Single Cell Imaging and Manipulation in Master Circadian Clock
OpenAlex ID: W2583510643