Biodegradative decolorization of Reactive Red 195-A by an isolated bacteria Staphylococcus sp: studies on metabolites and toxicity
Year: 2017
Type: article
Abstract: ABSTRACT The study is focussed on complete decolorization and degradation of an azo chromophore- based dye CI Reactive Red 195-A (RR 195-A) within 6 h of growth of a newly isolated bacterial species, Staphylococcus hominis subsp. hominis DSM 20328, under optimized conditions of pH 7, temperature 37°C and salt concentration (50 mgL –1 ). Induction of intracellular azoreductase, and NADH-DCIP reductase indicated their contribution in the biodegradation. The decolorization kinetics was studied and the kinetic parameters evaluated as rdye max = 19.158 mg L –1 h –1 and k m = 347.28 mgL –1 . Ring opening of the dye during biodegradation produced carbon source for the growth of the bacterium. The products of biodegradation were monitored using UV- vis spectroscopy and characterized using FTIR, HPLC and GCMS. The proposed metabolic pathway with respect to the GCMS analysis revealed the formation of naphthalene, naphthalen-1-ol, 1, 3, 5 triazinan-2-one, α – ketoglutaric acid, aniline and benzene. The toxicity studies, namely, phytotoxicity, microtoxicity and cytotoxicity revealed that the metabolites produced are less toxic as compared to the parent dye. The newly isolated bacterium is non-pathogenic and has exhibited the potential to decolorize and degrade different dyes that could be present in the textile effluent showing its usefulness for possible industrial application.
Source: Desalination and Water Treatment
Authors: Neha D. Parmar, Sanjeev R. Shukla
Institution: Institute of Chemical Technology
Language: English
Subfield: Applied Microbiology and Biotechnology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $1,000
OpenAlex ID: W2590420009