Work

Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy

Year: 2017

Type: article

Abstract: In this work, we investigated the effect of pH on Streptomyces M37 growth and its acarbose biosynthesis ability. We observed that low pH was beneficial for cell growth, whereas high pH favored acarbose synthesis. Moreover, addition of glucose and maltose to the fermentation medium after 72 h of cultivation promoted acarbose production. Based on these results, a two-stage fermentation strategy was developed to improve acarbose production. Accordingly, pH was kept at 7.0 during the first 72 h and switched to 8.0 after that. At the same time, glucose and maltose were fed to increase acarbose accumulation. With this strategy, we achieved an acarbose titer of 6210 mg/L, representing an 85.7% increase over traditional batch fermentation without pH control. Finally, we determined that the increased acarbose production was related to the high activity of glutamate dehydrogenase and glucose 6-phosphate dehydrogenase.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0166985

Authors: Fei Ren, Long Chen, Shuangli Xiong, Qunyi Tong

Institutions: Jiangnan University, Southwest University of Science and Technology, State Key Laboratory of Food Science and Technology

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Applied Microbiology and Biotechnology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,382

Funder: Priority Academic Program Development of Jiangsu Higher Education Institutions