Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits
Year: 2017
Type: article
Abstract: Using single-molecule approaches, we directly observed the dynamic interaction between HDAC8 and various ligands as well as conformational interconversions during the catalytic reaction. Statistical analysis identified key kinetic parameters, demonstrating that the enzymatic activity is highly sensitive to both minor variations in the ligand structures and small synthetic molecules.
Source: Chemical Communications
DOI: 10.1039/c6cc09949a
Authors: Seungyong You, James Froberg, Junru Yu, Manas Haldar, Abbas Sedigh, Sanku Mallik, D. K. Srivastava, Yongki Choi
Institution: North Dakota State University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2592839121