Year: 2017
Type: article
Abstract: Breast cancer (BC) remains the most prevalent oncologic pathology in women, causing huge psychological, economic and social impacts on our society. Currently, the available diagnostic tools have limited sensitivity and specificity. Metabolome analysis has emerged as a powerful tool for obtaining information about the biological processes that occur in organisms, and is a useful platform for discovering new biomarkers or make disease diagnosis using different biofluids. Volatile organic compounds (VOCs) from the headspace of cultured BC cells and normal human mammary epithelial cells, were collected by headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography combined with mass spectrometry (GC-MS), thus defining a volatile metabolomic signature. 2-Pentanone, 2-heptanone, 3-methyl-3-buten-1-ol, ethyl acetate, ethyl propanoate and 2-methyl butanoate were detected only in cultured BC cell lines. Multivariate statistical methods were used to verify the volatomic differences between BC cell lines and normal cells in order to find a set of specific VOCs that could be associated with BC, providing comprehensive insight into VOCs as potential cancer biomarkers. The establishment of the volatile fingerprint of BC cell lines presents a powerful approach to find endogenous VOCs that could be used to improve the BC diagnostic tools and explore the associated metabolomic pathways.
Source: Scientific Reports
DOI: 10.1038/srep43969
Institutions: Centro de Química da Madeira, Universidade da Madeira
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: G1514845986, G1878099885, G2192244390, G2292490070, EXPLORANDO O VOLATOMA DE DOENÇAS NÃO-TRANSMISSÍVEIS COMO UMA ABORDAGEM INOVADORA, PROMISSORA E INTEGRATIVA PARA O SEU RÁPIDO DIAGNÓSTICO. O CANCRO E AS DOENÇAS NEURODEGENERATIVAS COMO EXEMPLO DE APLICAÇÃO, From the development of a new nanomaterial-based device to the early disease diagnosis of lung cancer based on the volatomic and proteomic pattern of exhaled breath - Innovative stratey for selective removal of ethyl carbamate from wines through the development of a molecular imprinted polymer coated on celulose nanofibers, Non-invasive, innovative and promising strategy for early diagnosis of breast cancer through the establishment of urinary volatile metabolomic profiles of breast cancer patients as well as from breast cancer tissues and breast cancer cell lines, G6064036437
OpenAlex ID: W2594895839