A Cassette Containing Thiostrepton, Gentamicin Resistance Genes, and dif sequences Is Effective in Construction of Recombinant Mycobacteria
Year: 2017
Type: article
Abstract: The genetic manipulation of Mycobacterium tuberculosis genome is limited by the availability of selection markers. Spontaneous resistance mutation rate of M. tuberculosis to the widely used kanamycin is relatively high which often leads to some false positive transformants. Due to the few available markers we have created a cassette containing thiostrepton resistance gene (tsr) for selection in M. tuberculosis and M. bovis BCG, and gentamicin resistance gene (aacC1) for E.coli and M. smegmatis mc2155, flanked with dif sequences recognized by the Xer system of mycobacteria. This cassette adds to the limited available selection markers for mycobacteria.
Source: Frontiers in Microbiology
Institutions: Chinese Academy of Sciences, Guangzhou Institutes of Biomedicine and Health, State Key Laboratory of Respiratory Disease, University of Chinese Academy of Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Guangzhou Chest Hospital, Wuhan Institute of Virology, State Key Laboratory of Virology
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W2597921077