Metabolic Profiling of Cells in Response to Drug Treatment using 1H High-resolution Magic Angle Spinning (HR-MAS) NMR Spectroscopy
Year: 2017
Type: article
Abstract: High-resolution magic angle spinning (HR-MAS) is an NMR technique that provides access to well resolved liquid-like 1H NMR spectra of semi-solid samples. Therefore, 1H HR-MAS NMR spectroscopy has become an important tool for the direct analysis of biological samples such as tissues and cells in a mostly non-destructive way. Here, we focus on the application of HR-MAS NMR combined with multivariate statistical methods used for metabolic profiling of cells and in particular for the study of cellular metabolic responses to drug exposure. The principles of HR-MAS and the metabolomic approach are briefly described. As an example, a study on the metabolic response of different cell types towards treatment with a highly cytotoxic hexacationic ruthenium metallaprism as potential anti-cancer drug is presented. Specific metabolites and metabolic pathways are suggested to be associated with the cellular response. The study demonstrates the potential of HR-MAS metabolomics applied to cells for addressing the intracellular processes involved in the treatment with organometallic drugs.
Institutions: University of Bern, University Hospital of Bern
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
Awards: High Resolution Magic Angle Spinning (HR-MAS) probe for NMR Spectroscopic Investigations of semi-solid liquid-like materials, Advanced multi-modal MR Imaging and Spectroscopy for Comprehensive Characterization of Renal Function in Native and Transplanted Kidneys., Amino acid conjugated Photosensitizers in biocompatible nanoparticles and their interactions with Cellular Environments studied by High Resolution Liquid and Magic Angle Spinning (HR-MAS) NMR Spectroscopy
OpenAlex ID: W2603676906