Purification and Characterization of Polygalacturonase Produced by Aspergillus niger AN07 in Solid State Fermentation
Year: 2017
Type: article
Abstract: Polygalacturonase, an industrial enzyme has been produced from various fungal isolates using solid state and submerged fermentation techniques. The challenge has been yield, extraction and cost of production. In the present study, a low cost solid substrate, dried papaya peel was employed for polygalacturonase production using Aspergillus niger AN07. Polygalacturonase enzyme from Aspergillus niger AN07 was purified to 24.8 fold with a 52.6% recovery through anion exchange chromatography on DEAE-cellulose and gel filtration chromatography using Sephadex G-200. The SDS-PAGE revealed that the enzyme was monomeric with a molecular weight of 64.5 kDa. The optimum pH and temperature were 5.0 and 55℃, respectively. This enzyme was stable over a wide pH range (4.0-7.0) and relatively high temperature of 55℃ for 1 h. The Km and Vmax values of polygalacturonase for polygalacturonic acid were 2.6 mg/l and 181.8 µmol/ml/min, respectively. The purified enzyme could digest the polygalacturonic acid into oligosaccharides with a small amount of galacturonic acid as visualized on thin layer chromatography.
Institution: Devi Ahilya Vishwavidyalaya
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $250
OpenAlex ID: W2609268683