Use of green fluorescent protein for online, single cell detection of bacteria introduced into activated sludge microcosms
Year: 1998
Type: article
Abstract: A derivative of broad host-range plasmid RP4 was tagged with the green fluorescent protein (GFP) and this construct was used to mark various Gram-negative bacteria. Video recording of combined phase contrast and epifluorescence microscopy was employed to track the fate of individual marked cells after introduction into a sludge microcosm. Whether the introduced bacteria were laboratory strains or activated sludge isolates. they were found to be rapidly eliminated from the microcosms as a result of intense predation by protozoa. Furthermore, a method was developed that allows detection of GFP-tagged bacteria in fixed samples that are simultaneously used for in situ hybridisation with a eukaryote-specific rRNA-targeted oligonucleotide probe for visualisation of protozoa.
Source: TSpace (University of Toronto)
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2625835932