Work

Effect of osmolarity and medium composition on callogenesis, caulogenesis and rhizogenesis ofBrassica oleracea L. var.botrytis hypocotyl fragments

Year: 1993

Type: article

Abstract: Callus formation by fragments of cauliflower hypocotyls was favoured by raising culture medium osmolarity above -0.38 MPa. Increase in sucrose concentration while diminishing macronutrients inhibited callus initiation and growth. Root formation by the same material required a low medium osmolarity (-0.19 MPa). Reducing sucrose concentration in the classical Murashige and Skoog rooting culture medium favoured root formation. Adventitious bud formation was also depending upon medium osmolarity besides the need for a cytokinin. Reducing too much the osmotic potential of the medium had an unfavourable effect on bud neoformation. The importance of sucrose in all these processes is pointed out.

Source: Biologia Plantarum

DOI: 10.1007/bf02921113

Authors: J. L. Vandemoortele, J. P. Billard, J. Boucaud, T. Gaspar

Institutions: Estación Experimental de Pastos y Forrajes Indio Hatuey, Université de Caen Normandie, University of Liège

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

OpenAlex ID: W263384663