Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation
Year: 2017
Type: article
Abstract: Programmable sequence-specific genome editing agents such as CRISPR-Cas9 have greatly advanced our ability to manipulate the human genome. Although canonical forms of genome-editing agents and programmable transcriptional regulators are constitutively active, precise temporal and spatial control over genome editing and transcriptional regulation activities would enable the more selective and potentially safer use of these powerful technologies. Here, by incorporating ligand-responsive self-cleaving catalytic RNAs (aptazymes) into guide RNAs, we developed a set of aptazyme-embedded guide RNAs that enable small molecule-controlled nuclease-mediated genome editing and small molecule-controlled base editing, as well as small molecule-dependent transcriptional activation in mammalian cells.
Source: Nature Communications
DOI: 10.1038/ncomms15939
Authors: Weixin Tang, Johnny H. Hu, David R. Liu
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Funders: Howard Hughes Medical Institute, Jane Coffin Childs Memorial Fund for Medical Research, Yale University, Harvard University, National Institutes of Health, Defense Advanced Research Projects Agency, National Defense Science and Engineering Graduate, National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering
OpenAlex ID: W2725317476