Work

Prenatal developmental toxicity testing of petroleum substances: Application of the mouse embryonic stem cell test (EST) to compare in vitro potencies with potencies observed in vivo

Year: 2017

Type: article

Abstract: Prenatal developmental toxicity (PDT) as observed with some petroleum substances (PS) has been associated with the presence of 3–7 ring polycyclic aromatic hydrocarbons (PAHs). In the present study, the applicability of ES-D3 cell differentiation assay of the EST to evaluate in vitro embryotoxicity potencies of PS and gas-to-liquid (GTL) products as compared to their in vivo potencies was investigated. DMSO-extracts of a range of PS, containing different amounts of PAHs, and GTL-products, which are devoid of PAHs, were tested in the ES-D3 cell proliferation and differentiation assays of the EST. The results show that PS inhibited the differentiation of ES-D3 cells into cardiomyocytes in a concentration-dependent manner at non-cytotoxic concentrations, and that their potency was proportional to their PAH content. In contrast, as expected, GTL-products did not inhibit ES-D3 cell viability or differentiation at all. The in vitro PDT potencies were compared to published in vivo PDT studies, and a good correlation was found between in vitro and in vivo results (R2 = 0.97). To conclude, our results support the hypothesis that PAHs are the primary inducers of the PDT in PS.

Source: Toxicology in Vitro

DOI: 10.1016/j.tiv.2017.07.018

Authors: Lenny Kamelia, Jochem Louisse, Laura de Haan, Ivonne M.C.M. Rietjens, Peter J. Boogaard

Institutions: Wageningen University & Research, Shell (Netherlands)

Language: English

Topic: Animal testing and alternatives

Subfield: Small Animals

Field: Veterinary

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,200