Optimization of surfactant-mediated, ultrasonic-assisted extraction of antioxidant polyphenols from rattan tea (Ampelopsis grossedentata) using response surface methodology
Year: 2017
Type: article
Abstract: Background: Rattan tea is a medicinal plant that has been used for many years for the treatment of inflammation, fatty liver, tumor, diabetes, and hyperlipidemia.Objective: A green and novel approach based on surfactant-mediated, ultrasonic-assisted extraction (SM-UAE) was developed for the extraction of antioxidant polyphenols from Rattan tea.A nonionic surfactant Tween-80 was selected as extraction solvent.The antioxidant activity was measured by total phenolic content (TPC) and ferric-reducing/antioxidant capacity (FRAC) assay.Materials and Methods: Optimization of extraction parameters including concentration of solvent, ultrasonic time, and temperature were investigated by response surface methodology.The antioxidant activity was measured by TPC and FRAC assay.Results: The optimal extraction conditions were determined as 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54°C, and ultrasonic time of 28.8 min.Under these conditions, the highest TPC value of 360.4 mg gallic acid equivalent per gram of dry weight material (GAE/g DW) was recorded.Moreover, 6.8% (v/v) of aqueous Tween-80, ultrasonic temperature of 54.5°C, and ultrasonic time of 28.4 min were determined for the highest FRAC value of 478.2 μmol of Fe 2+ /g of weight material (μmol Fe 2+ /g DW).Compared with other methods, the TPC and FRAC values of 313.5 mg GAE/g DW and 389.6 μmol Fe 2+ /g DW were obtained by heat reflux extraction using ethanol as solvent, respectively, and 343.2 mg GAE/g DW and 450.1 μmol Fe 2+ /g DW were obtained by UAE using ethanol as solvent, respectively.Conclusion: The application of SM-UAE markedly decreased extraction time or extraction cost and improved the extraction efficiency, compared with the other methods.
Source: Pharmacognosy Magazine
DOI: 10.4103/pm.pm_159_16
Authors: Xiu-Quan Xu, Feng Li, Aun Raza, Yan-Wei Wang, Guan-Hua Chen
Institutions: Jiangsu University, Affiliated Hospital of Jiangsu University
Language: English
Subfield: Biochemistry (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2736848331