Ultrasound-assisted lipase catalyzed hydrolysis of aspirin methyl ester

Year: 2017

Type: article

Abstract: The ultrasound-assisted hydrolysis of aspirin methyl ester (AME) was investigated using immobilized Candida antarctica lipase B (CALB) (1%) in the presence of solvents like triolein, chloroform (CHCl 3 ) and dichloromethane (DCM). The effect of ultrasound and the role of water on the conversion rates have also been investigated. Proton nuclear magnetic resonance spectroscopic ( 1 H NMR) was chosen to calculate hydrolysis convertion rates. We observed that lipase-ultrasound assisted hydrolysis of AME in the presence of triolein and water showed the highest hydrolysis conversion rate (65.3%). Herein low water amount played an important role as a nucleophile being crucial for the hydrolysis yields obtained. Lipase activity was affected by the conjugated action of ultrasound and solvents (35.75% of decrease), however not disturbing its hydrolytic efficiency. It was demonstrated that lipase is able to hydrolyse AME to methyl 2-hydroxy benzoate (methyl salicylate), which applications include fragrance agents in food, beverages and cosmetics, or analgesic agent in liniments.

Source: Ultrasonics Sonochemistry

DOI: 10.1016/j.ultsonch.2017.08.004

Authors: Pranali P. Chiplunkar, Xiaoman Zhao, Prerana D. Tomke, Jennifer Noro, Bo Xu, Qiang Wang, Carla Silva, Amit P. Pratap, Artur Cavaco-Paulo

Institutions: Jiangnan University, Institute of Chemical Technology, University of Minho

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,710

Funders: Government of Jiangsu Province, Jiangnan University, Universidade do Minho, Fundação para a Ciência e a Tecnologia, European Regional Development Fund, Fundamental Research Funds for the Central Universities

OpenAlex ID: W2742829006