Work

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Year: 2017

Type: article

Abstract: The goal of this research is to develop a single-walled carbon nanotube- (SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference ( ΔR ) was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor ( P<0.05 ). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 log⁡CFU/mL .

Source: Journal of Food Quality

DOI: 10.1155/2017/5239487

Authors: Hyun-Kyung Choi, Jinyoung Lee, Mi-Kyung Park, Jun-Hyun Oh

Institutions: Sangmyung University, Kyungpook National University

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,570