Work

Mandibular Explant Assay for Investigating Extrinsic Stimuli on Bone and Cartilage Development

Year: 2017

Type: article

Abstract: conditions. In this protocol, the explant model was applied to test whether Meckel's cartilage and surrounding tissues are responsive to the Endothelin1 (Edn1) signaling molecule and whether it would rescue the phenotype of genetic mutations. The system allows a high degree of manipulation in terms of the concentrations of exogenous compounds added to the explant, time points with regards to measuring mandibular development, and the method of application of exogenous molecules and teratogens.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2641

Authors: Jamie Jiang, Jessica Bertol, Walid Fakhouri

Institution: The University of Texas Health Science Center at Houston

Language: English

Topic: Craniofacial Disorders and Treatments

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of General Medical Sciences